HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5

HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5

Conditions

column Discovery HS F5, 5 cm x 4.6 mm I.D., 5 µm particles (567513-U)
column temp. 40 °C
mobile phase [A] 10 mM ammonium acetate, pH 6.8 (unadjusted); [B] acetonitrile
gradient 0% B for 1 min, to 30% B in 4 min, held 3 min
flow rate 1 mL/min
sample 50 µg/mL in final mobile phase
injection 10 µL
detector UV, 280 nm

Description

Categories Analytical Chromatography, Clinical, drugs of abuse, Drugs of Abuse
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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