Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Mouse Ascites Fluid on TSKgel® BioAssist® Q vs. Competitive Q Column

HPLC Analysis of Mouse Ascites Fluid on TSKgel® BioAssist® Q vs. Competitive Q Column

Conditions

column TSKgel BioAssist Q, 5 cm x 4.6 mm I.D., 10 µm particles (819685)
column temp. 25 C
gradient 15 min linear gradient of NaCl from 0 to 1 M in 20 mM Tris-HCl buffer, pH 8.0
flow rate 1 ml/min
sample mouse ascites fluid (3-fold dilution in 20 mM Tris-HCl buffer, pH 8.0)
injection 5 µl
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BioAssist is a registered trademark of Tosoh Corporation, TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
Related Links