HPLC Analysis of Nitroaromatic Compounds Using Ascentis® Express Biphenyl

HPLC Analysis of Nitroaromatic Compounds Using Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 10 cm x 2.1mm I.D., 2.7 µm particles (64065-U)
column temp. 35 C
mobile phase [A]:Water; [B]:Methanol; (50:50, A:B)
flow rate 0.5 ml/min
pressure 7150 psi (492 bar)
sample Toluene, Nitrobenzene;1,3,5-Trinitrobenzene; 2,4-Dinitrotoluene.  100 μg/mL in 50:50 A:B
injection 1 µl
detector UV, 254 nm

Description

Analysis Note This application demonstrates the separation of selected nitroaromatic compound using Ascentis Express Biphenyl. The biphenyl stationary phase is well suited to these substituted aromatic compounds due to interactions between the aromatic stationary phase and the electropositive benzene ring of the analyte. Unique selectivity is observed running this reversed phase separation using methanol mobile phase conditions
Categories Analytical Chromatography, Aromatics, Explosives
Featured Industry Forensics and Toxicology
General Analytical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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