HPLC Analysis of Nonvolatile Pesticides in Water on SUPELCOSIL™ LC-18-DB after SPE using Supelclean™ ENVI-Carb™

HPLC Analysis of Nonvolatile Pesticides in Water on SUPELCOSIL™ LC-18-DB after SPE using Supelclean™ ENVI-Carb™

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix water spiked with pesticides at 10-50 µg/L
SPE tube/cartridge Supelclean ENVI-Carb, 250 mg/3 mL (57088)
condition 5 mL methylene chloride:methanol (80:20); 1 mL methanol; 10 mL 2% acetic acid in water (keep bed moist until sample addition)
sample addition 100 mL sample at 5 mL/min
drying 1 minute with vacuum
elution 0.8 mL methanol; 2 x 35 mL methylene chloride:methanol (80:20)
eluate post-treatment dry eluate to 500 µL under gentle nitrogen stream, reconstitute to 1 mL with methanol
column SUPELCOSIL LC-18-DB, 25 cm x 4.6 mm I.D., 5 µm particles (58355-U)
mobile phase [A] water:acetonitrile (90:10), [B] acetonitrile; gradient 80% A for 5 min then to 30% A over 30 min
flow rate 1.5 mL/min
injection 20 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Environmental Analysis, SPE Applications, Food & Beverage Analysis, Pesticides, Organics, Pesticide and Metabolite Residues, Chromatograms, Applications (Chromatograms), HPLC Chromatograms
Featured Industry Environmental
Legal Information ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC, Supelclean is a trademark of Sigma-Aldrich Co. LLC, SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
Related Links