HPLC Analysis of Norepinephrine and 3,4-Dihydroxyphenylglycol on Discovery® HS F5

HPLC Analysis of Norepinephrine and 3,4-Dihydroxyphenylglycol on Discovery® HS F5

Conditions

column Discovery HS F5, 15 cm x 4.6 mm I.D., 5 µm particles (567561-U)
column temp. 35 C
mobile phase 50 mM ammonium formate, pH to 3.0 with formic acid
flow rate 1.0 ml/min
sample 50 µg/mL each (NE and DHPG) in 5% methanol in mobile phase
injection 10 µl
detector UV, 266 nm

Description

Categories Analytical Chromatography, Clinical, Hormones, hormones
Featured Industry Clinical
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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