Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Nucleosides on Ascentis® Si (Silica)

HPLC Analysis of Nucleosides on Ascentis® Si (Silica)

Conditions

column Ascentis Si, 10 cm x 2.1 mm I.D., 5 µm particles (581508-U)
column temp. 35 C
mobile phase [A] 150 mM ammonium formate (pH 3.0, with formic acid): [B] acetonitrile; (10:90, A:B)
flow rate 0.2 ml/min
sample 100 µg/mL in mobile phase
injection 1 µl
detector UV, 270 nm

Description

Categories Analytical Chromatography, Nucleic Acids, Nucleosides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links