HPLC Analysis of Nucleosides on Ascentis® Phenyl

HPLC Analysis of Nucleosides on Ascentis® Phenyl

Conditions

column Ascentis Phenyl, 15 cm x 2.1 mm I.D., 5 µm particles (581613-U)
column temp. 35 °C
mobile phase 10 mM ammonium formate (pH 3.0 with formic acid)
flow rate 0.2 mL/min
sample as indicated, in mobile phase
injection 1 µL
detector UV, 270 nm; 750 psi back pressure regulator on outlet of flow cell

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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