HPLC Analysis of Oenothein B on Ascentis® Express RP-Amide, UV and MS Detection

HPLC Analysis of Oenothein B on Ascentis® Express RP-Amide, UV and MS Detection

Conditions

column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 µm particles (53929-U)
column temp. 35 °C
mobile phase [A] 0.2% formic acid; [B] methanol
gradient 5% B for 2 min; to 90% B over 18 min; held at 90% B for 10 min
flow rate 0.6 mL/min
sample 1 mg/mL in methanol
injection 2 µL
detector UV, 254 nm
detector MS (ion trap), m/z 100-1500

Description

Analysis Note Optimization of a published method showing much better retention, peak shape and resolution on Supelco Ascentis Express RP-Amide 2.7 μm compared to a C18 phase. Isomers and small amounts of homologues are resolved and detectable with higher sensitivity.
Categories Analytical Chromatography, Clinical, antineoplastics, Antineoplastics, antioxidants, herbal medicines, Antioxidants
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links