HPLC Analysis of Organic Acids in Beverages on Chromolith® HighResolution RP-18 endcapped

HPLC Analysis of Organic Acids in Beverages on Chromolith® HighResolution RP-18 endcapped

Conditions

sample preparation sonicate 5 mL of orange drink for 5 minutes, add 0.2 mL of phosphoric acid solution (1M), dilute to 10 mL with water
column Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 µm particles (1.52022)
column temp. 30 °C
mobile phase 10 mM diammonium hydrogen phosphate, pH 2.7
flow rate 1.0 mL/min
pressure 32 bar (464 psi)
injection 20 µL
detector UV, 210 nm

Description

Categories Analytical Chromatography, Organic Acids
Featured Industry Food and Beverages
General description Column Information: 1.52022.0001
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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