Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Pain Relievers on Ascentis® Express C18

HPLC Analysis of Pain Relievers on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 4.6 mm I.D., 5 µm particles (50536-U)
column temp. 45 C
mobile phase [A] water with 3% acetic acid; [B] methanol with 3% acetic acid; (79:21, A:B)
flow rate 2.0 ml/min
pressure 3290 psi (227 bar)
sample 100 - 360 µg/mLin mobile phase
injection 10 µl
detector UV,275 nm

Description

Analysis Note This application shows the improvements in efficiency that may be obtained for USP methods using modern Fused-Core technology.
Categories Analytical Chromatography, Clinical, analgesics, Analgesics
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links