HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)

Conditions

column Discovery BIO Wide Pore C18, 15cm x 4.6mm I.D., 5mum particles (568222-U)
column temp. 30 °C
mobile phase [A] (80:20), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile
gradient 0-100% B in 14 min after 1 minute delay
flow rate 1 mL/min
sample Sigma Peptide Mix (Cat. No. P2693)
injection 10 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links