HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (5 cm Column)

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (5 cm Column)

Conditions

column Discovery BIO Wide Pore C18, 5 cm x 4.6 mm I.D., 5 µm particles (568220-U)
column temp. 70 °C
mobile phase [A] (5:95), 0.5% TFA in water:0.5% TFA in acetonitrile; [B] (25:75), 0.5% TFA in water:0.5% TFA in acetonitrile
gradient 2-24% B in 22 min, 8 min. at 100%A
flow rate 2 mL/min
detector UV, 220 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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