HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (PFPA Mobile Phase Additive)

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (PFPA Mobile Phase Additive)

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 µm particles (568222-U)
column temp. 30 C
mobile phase [A] 81:19, (water/0.1% PFPA):(acetonitrile/0.1%PFPA); [B] 62:38, (water/0.1%PFPA):(acetonitrile/0.1%PFPA)
flow rate 1.0 ml/min
sample Sigma Peptide Mix (Cat. No. P2693)
injection 10 µl (~25 mug each peptide)
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links