HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 µm particles (568222-U)
column temp. 30 °C
mobile phase [A] (95:5), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (50:50), 0.1%TFA in water:0.1% TFA in acetonitrile
gradient 0-100% B in 65 min
flow rate 1 mL/min
injection 50µL carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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