HPLC Analysis of Phenylacetic Acid on Ascentis® C18

HPLC Analysis of Phenylacetic Acid on Ascentis® C18

Conditions

column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 °C
mobile phase [A] 20 mM phosphoric acid; [B] acetonitrile; (75:25, A:B)
flow rate 1.0 mL/min
sample 50 µg/mL in water: acetonitrile: methanol (75: 20: 5)
injection 5 µL
detector UV, 215 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis C18 for the analysis of phenylacetic acid.
Categories Analytical Chromatography, Drugs of Abuse, Organic Acids
Featured Industry Chemicals and Industrial Polymers
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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