HPLC Analysis of Pilocarpine, Betaxolol, and Benzalkonium Chloride on Ascentis® Express HILIC

HPLC Analysis of Pilocarpine, Betaxolol, and Benzalkonium Chloride on Ascentis® Express HILIC

Conditions

column Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 µm particles (53981-U)
column temp. 35 °C
mobile phase [A] 20 mM ammonium acetate; [B] acetonitrile; (10:90, A:B)
flow rate 1 mL/min
sample 10 mg/L in acetonitrile
injection 2 µL
detector ESI (+), XIC m/z 304, 332 (BAC), 209 (pilocarpine), 308 (betaxolol)

Description

Categories Analytical Chromatography, Alkaloids, Antihypertensives, Surfactants
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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