HPLC Analysis of Polar Neutral and Acidic Amino Acids on Ascentis® Si (Silica)

HPLC Analysis of Polar Neutral and Acidic Amino Acids on Ascentis® Si (Silica)

Conditions

column Ascentis Si, 10 cm x 2.1 mm I.D., 5 µm particles (581508-U)
column temp. 35 °C
mobile phase [A] 100 mM ammonium formate, pH 3.0, with formic acid; [B] water, [C] acetonitrile
gradient 5% A constant; 10% B, 85% C to 16% B, 79% C in 20 min
flow rate 0.3 mL/min
sample 100 µg/mL in 10:90, (50 mM ammonium formate / formic acid, pH 3.0):acetonitrile
injection 2 µL
detector ESI (+), full scan

Description

Categories Analytical Chromatography, Amino Acids
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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