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HPLC Analysis of Prednisolone (Secondary Pharma Standard) on Ascentis® C18

HPLC Analysis of Prednisolone (Secondary Pharma Standard) on Ascentis® C18

Conditions

column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 40 C
mobile phase [A] water; [B] methanol:acetonitrile (50:50, A:B)
gradient 40% B for 14 min; to 80% B in 6 min; held at 80% B for 5 min
flow rate 1 ml/min
sample 125 µg/mL in water:acetonitrile (60:40)
injection 10 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Steroids
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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