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HPLC Analysis of Procainamide-Labeled Human IgG Glycans on BIOshell™ Glycan using HILIC-FLR

HPLC Analysis of Procainamide-Labeled Human IgG Glycans on BIOshell™ Glycan using HILIC-FLR

Conditions

column BIOshell Glycan, 15 cm x 2.1 mm I.D., 2.7 µm particles (50994-U)
column temp. 58 C
mobile phase [A] 50 mM ammonium formate, pH 4.4 (50 mM ammonium hydroxide, adjusted to pH 4.4 with formic acid); [B] acetonitrile
gradient 75 to 59% B in 75 min
flow rate 0.3 ml/min
sample human IgG
injection 10 µl
detector FLR, ex 308 nm, em 359 nm

Description

Analysis Note A sample of human IgG glycans was analyzed on a BIOshell Glycan HPLC Column resulting in the identification of 20 distinct peaks. Excellent separation as well as symmetrical peak shape can be observed in the chromatogram. This chromatogram shows 55 minutes of an 80 minute analysis
Categories Analytical Chromatography, Carbohydrates
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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