HPLC Analysis of Promethazine and Degradants on Ascentis® Phenyl

HPLC Analysis of Promethazine and Degradants on Ascentis® Phenyl

Conditions

column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 C
mobile phase [A] 0.1% ammonium acetate: [B] methanol; (25:75, A:B)
flow rate 1.0 ml/min
sample 50 µg/mL in 20:80, water:methanol
injection 10 µl
detector UV, 220 nm

Description

Categories Analytical Chromatography, Antihistamines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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