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HPLC Analysis of Protein Standards on Zenix® SEC-300 in the Presence of a Denaturant

HPLC Analysis of Protein Standards on Zenix® SEC-300 in the Presence of a Denaturant

Conditions

column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777028)
column temp. 25 C
mobile phase [A] 0.1 M ammonium bicarbonate (pH unadjusted); [B] 0.1 M ammonium bicarbonate (pH unadjusted) with 6 M guandine hydrochloride (pH unadjusted)
flow rate 0.3 ml/min
pressure 1300 psi (90 bar)
sample Thyroglogulin, 1.5 g/L; IgG, 1 g/L; Albumin, 2.5 g/L; Myoglobin, 1 g/L; Uracil, 0.05 g/L; in mobile phase
injection 2.5 µl
detector UV, 280 nm

Description

Analysis Note This application compares selectivity of proteins when chromotographed by size exclusion in the presence and absence of a denaturant (guandine-HCl).
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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