HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW

HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW

Conditions

column TSKgel Chelate-5PW, 5 cm x 5 mm I.D., 10 µm particles (column saturated with Zn2+ ions) (814440)
mobile phase [A] 20 mM HEPES, 0.5 M sodium chloride, 1 mM imidazole, pH 8 with sodium hydroxide; [B]  20 mM HEPES, 0.5 M sodium chloride, 20 mM imidazole, pH 8 with sodium hydroxide
gradient 30 min linear gradient from 1 mM to 20 mM imidazole in 20 mM HEPES-NaOH buffer, pH 8 containing 0.5 M NaCl
flow rate 0.8 mL/min
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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