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HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5

HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5

Conditions

column Proteomix WCX-NP5, 5 cm x 4.6 mm I.D., 5 µm particles (Z777193)
column temp. 25 C
mobile phase [A] 10 mM potassium phosphate, dibasic, pH 7 with phosphoric acid; [B] 0.3 M potassium potassium phosphate, pH 7 with phosphoric acid; ([C] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [D] 0.3 M sodium potassium phosphate, dibasic, pH 7 with phosphoric acid;)
gradient 100% A to 100% B (or 100% C to 100% D) in 20 min
flow rate 0.5 ml/min
pressure 615 psitotal pressure (42 bar total pressure)
sample g/L ea: ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23
injection 5 µl
detector UV, 280 nm

Description

Analysis Note The choice of exchangable counterion can affect retention and selectivity with ion exchange chromatography of proteins. In this case, under the chromatographic conditions employed, differences are subtle.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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