HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers

HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers

Conditions

column TSKgel SP-5PW, 7.5 cm x 7.5 mm I.D., 5 µm particles (807161)
column TSKgel CM-5PW, 7.5 cm x 7.5 mm I.D., 10 µm particles (813068)
mobile phase [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl pH 7.0
gradient 0 to 100% B in 60 min
flow rate 1 ml/min
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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