HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution

HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution

Conditions

column Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 µm particles (Z777151)
column Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 µm particles (Z777146)
column temp. Ambient
mobile phase (A) 20 mM sodium phosphate buffer, pH 6.0, (B) A + 1.0 M NaCl
gradient 0-25 min from 0-75% B, 15 min prewash
flow rate 0.75 mL/min
sample each 1 mg/mL
injection 5 µL
detector 214 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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