HPLC Analysis of Proteins on BIOshell™ A400 Protein C4, Rapid Separation at Elevated Temperature

HPLC Analysis of Proteins on BIOshell™ A400 Protein C4, Rapid Separation at Elevated Temperature

Conditions

column BIOshell A400 Protein C4, 5 cm x 2.1 mm I.D., 3.4 µm particles (66824-U)
column temp. 90 °C
mobile phase [A] 75:25 (0.1% TFA in water):(0.1% TFA in acetonitrile); [B] 25:75 (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient 12 to 100% B in 1 min; held at 100% B for 1 min
flow rate 0.4 mL/min
pressure 905 psi (62 bar)
sample protein mix in 0.05% TFA
injection 1 µL
detector UV, 215 nm

Description

Analysis Note This application demonstrates the suitability of BIOshell A400 Protein C4 column for fast elution of proteins via ballistic gradients. High temperature enhances chromatographic performance and lowers operational backpressure. The highest grade solvents provided clean, robust operation.
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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