HPLC Analysis of Proteins on TSKgel® BioAssist S

HPLC Analysis of Proteins on TSKgel® BioAssist S

Conditions

column TSKgel BioAssist S, 5 cm x 4.6 mm I.D., 7 µm particles (819686)
column temp. 25 C
mobile phase [A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
gradient 0 to 60% in 20 min, 60 to 100% in 0.5 min, held for 5 minutes
flow rate 0.3 ml/min
pressure 400 psitotal pressure (28 bar)
sample ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
injection 5 µl
detector UV, 280 nm

Description

Analysis Note Four commonly investigated proteins were chromatographed on a TSKgel BioAssist S cation exchange column. This column was found to provide better resolution than the Proteomix WCX-NP5, but less resolution between the proteins than the Proteomics SCX-NP5.
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
suitability application for HPLC

Materials

     
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