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HPLC Analysis of Proteins on Proteomix® WCX-NP5

HPLC Analysis of Proteins on Proteomix® WCX-NP5


column Proteomix WCX-NP5, 5 cm x 4.6 mm I.D., 5 µm particles (Z777193)
column temp. 25 C
mobile phase [A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
gradient 0 to 60% in 20 min
flow rate 0.3 ml/min
pressure 420 psitotal pressure (29 bar)
sample ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
injection 5 µl
detector UV, 280 nm


Analysis Note Four commonly studied proteins were chromatographed using a Proteomics WCX-NP5 column to examine retention time and resolution. It was found that the Proteomics WCX-NP5 column does not resolve the proteins as well as the Proteomics SCX-NP5, strong cation exchange column.
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC


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