Certain features of will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Proteins on Proteomix® SCX-NP5

HPLC Analysis of Proteins on Proteomix® SCX-NP5


column Proteomix SCX-NP5, 5 cm x 4.6 mm I.D., 5 µm particles (Z777159)
column temp. 25 C
mobile phase [A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
gradient 0 to 60% in 20 min
flow rate 0.3 ml/min
pressure 400 psitotal pressure (28 bar)
sample ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
injection 5 µl
detector UV, 250 nm


Analysis Note The separation of four readily investigated proteins was examined on a Proteomix SCX-NP5 column in order to examine retention time and resolution. The four peaks were well resolved in under 15 minutes.
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC


Related Links