HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns

HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns

Conditions

column TSKgel Phenyl-5PW, 7.5 cm x 7.5 mm I.D., 10 µm particles (807573)
column TSKgel Butyl-NPR, 3.5 cm x 4.6 mm I.D., 2.5 µm particles (814947)
column TSKgel Ether-5PW, 7.5 cm x 7.5 mm I.D., 10 µm particles (808641)
mobile phase for 5PW columns: [A] 1.8 M ammonium sulfate, 0.1 M phosphate buffer, pH 7.0; [B] 0.1 M phosphate buffer, pH 7.0
mobile phase for Butyl-NPR column: [A] 2.3 M ammonium sulfate, 0.1 M phosphate buffer, pH 7.0; [B] 0.1 M phosphate buffer, pH 7.0
gradient for Butyl-NPR column: 0 to 100% B in 12 min
gradient for 5PW columns: 0 to 100% B in 60 min
flow rate 1 mL/min
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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