HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis® Express OH5

HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis® Express OH5

Conditions

column Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 µm particles (53769-U)
column temp. 35 °C
mobile phase 2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 7.0 with acetic acid, glacial
flow rate 0.5 mL/min
pressure 850 psi (59 bar)
sample 100 µg/mL salicylic acid, 200 µg/mL acetylsalicylic acid in methanol:acetonitrile (3:7)
injection 2 µL
detector UV, 235 nm

Description

Categories Analytical Chromatography, Analgesics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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