HPLC Analysis of Separation of Low MW Polysaccharides on SRT®

HPLC Analysis of Separation of Low MW Polysaccharides on SRT®

Conditions

column SRT SEC-150 30 cm x 4.6 mm I.D., 5 µm particles, 150 Ã… (Z777043)
column SRT SEC-100 30 cm x 4.6 mm I.D., 5 µm particles, 100 Ã… (Z777037)
mobile phase 0.15 M phosphate buffer, pH 7
flow rate 0.35 mL/min
sample Achyranthes bidentata polysaccharides (ABPS) from plant root extract (MW < 10,000)
injection 10 µL
detector UV, 214 nm

Description

Analysis Note Achyranthes bidentata polysaccharides (ABPS) is extracted from the root of a Chinese medicinal herb. ABPS acts as an immune modulator. It is tested for use as a replacement of antibiotics added to feedstock to protect piglets from microorganisms. ABPS contains mainly fructose and glucose units. The chromatograms show that the smallest pore size column, SRT SEC-100, provides the largest number of peaks, which is not unexpected given the low molecular mass (< 10,000 Da) of the sample.
Note that since ABPS does not carry an electrical charge, a 15 mM buffer concentration is sufficient to prevent interaction of the sample with the underlying silica surface.
Categories Analytical Chromatography, Polysaccharides
Featured Industry Life Science and Biopharma
Legal Information SRT is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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