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HPLC Analysis of Serotonin Metabolites on Ascentis® C18

HPLC Analysis of Serotonin Metabolites on Ascentis® C18


column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 C
mobile phase [A] 10 mM formic acid/acetate (pH 3.0): [B] methanol; (80:20, A:B)
flow rate 0.7 ml/min
sample 25 mg/L in 90:10, 10 mM formic acid/acetate (pH 3.0):methanol
injection 10 µl
detector ESI (+); overlay of extracted ion chromatograms of individual (M+H)+ species


Categories Analytical Chromatography, Clinical, hormones, Amines
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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