HPLC Analysis of Serotonin Synthetic Intermediates and Metabolites on Discovery® HS F5

HPLC Analysis of Serotonin Synthetic Intermediates and Metabolites on Discovery® HS F5

Conditions

column Discovery HS F5, 15 cm x 4.6 mm A.D., 5 µm particles (567516-U)
column temp. 35 C
mobile phase [A] water (10 mM ammonium sulfate, pH 3.0 with formic acid): [B]acetonitrile; (80:20, A:B)
flow rate 1.0 ml/min
sample 50 µg/mL each
injection 10 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Clinical, hormones, Amines
Featured Industry Clinical
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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