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HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V

HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V

Conditions

column CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 µm particles (11024AST)
column temp. 25 C
mobile phase 15 mM ammonium formate in methanol
flow rate 1 ml/min
sample 1 mg/mL in methanol
injection 5 µl
detector UV, 230 nm

Description

Analysis Note Sibutramine, one of a few drugs approved for long-term treatment of obesity, is a chiral compound. The (R) enantiomer has the desired effect of reducing body weight and food intake, but the (S) enantiomer has the opposite effect: A perfect example of why chiral HPLC is important to pharmaceutical and clinical analysts. Astec® CHIROBIOTIC® V successfully separates sibutramine enantiomers, using a simple, MS-friendly mobile phase.
Categories Analytical Chromatography, Chiral Applications, Pharmaceutical (small molecule), Antiobesity Drugs, All Pharmaceutical Applications
Featured Industry Pharmaceutical (small molecule)
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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