HPLC Analysis of Sigma Protein Standard Mix on Zenix® SEC-150

HPLC Analysis of Sigma Protein Standard Mix on Zenix® SEC-150

Conditions

column Sepax Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 µm particles (Z777016)
column temp. 25 C
mobile phase 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate 0.3 ml/min
pressure 1175 psi (81 bar)
sample 0.5 mL of mobile phase added to one vial of  Sigma Protein Standard Mix; filtered 0.45 µm Millex-HV syringe filter
injection 2.5 µl
detector UV, 215 & 280 nm

Description

Analysis Note Protein standards spanning a wide range of molecular weights can reveal the size range best resolved by a given gel filtration column. The Sepax 150Ã… SEC column provides good resolution of sample components ranging in molecular weight of <10,000 to ~150,000.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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