Certain features of will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Silymarin on Ascentis® Express C18

HPLC Analysis of Silymarin on Ascentis® Express C18


column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U)
column temp. 35 C
mobile phase [A] water with 0.1% formic acid; [B] methanol
gradient held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate 0.6 ml/min
pressure 4105 psi (283 bar)
sample 100 µg/mL in 90:10, water:methanol
injection 20 µl
detector UV, 254 nm


Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of silymarin.
Categories Analytical Chromatography, Phenols
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


Related Links