HPLC Analysis of Simple Underivatized Sugars on apHera™ NH2

HPLC Analysis of Simple Underivatized Sugars on apHera™ NH2

Conditions

column apHera NH2, 15 cm x 4.6 mm I.D., 5 µm particles (56401AST)
column temp. 25 C
mobile phase [A] water: [B] acetonitrile; (20:80, A:B)
flow rate 1.0 ml/min
sample 500 µg/mL in 30:70, water: acetonitrile
injection 10 µl
detector ELSD, 45 C, 3.5 psi nitrogen

Description

Categories Analytical Chromatography, Carbohydrates
Featured Industry Food and Beverages
Legal Information apHera is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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