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HPLC Analysis of Spice Cannabinoids JWH-018, JWH-073 and Metabolites on Ascentis® Express C18

HPLC Analysis of Spice Cannabinoids JWH-018, JWH-073 and Metabolites on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in water:acetonitrile (10:90)
gradient 20% B held for 3 min; 20 to 100% B in 0.1 min; 100% B held for 2 min
flow rate 0.5 ml/min
pressure 2150 psi (148 bar)
sample 17 µg/mL each in acetonitrile
injection 2 µl
detector UV, 315 nm

Description

Analysis Note Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. A rapid separation of six of these compounds on an Ascentis Express C18 column is shown here. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, drugs of abuse, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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