HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB

HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix rat plasma spiked with provastin and atorvastin at 5 and 100 ng/mL
SPE tube/cartridge Supel-Select HLB SPE, 30 mg/1 mL (54181-U)
condition 0.5 mL methanol:acetonitrile (1:1, v/v); 0.5 mL DI water
sample addition 0.5 mL
washing 0.5 mL 5% methanol
elution 0.5 mL methanol:acetonitrile (1:1, v/v)
eluate post-treatment evaporate to dryness with nitrogen at 37 C, reconstitute in 0.5 mL DI water
column Ascentis Express C18, 5 cm x 2.1 mm, 2.7 µm particles (53822-U)
column temp. 35 C
mobile phase 0.1% acetic acid diluted in 60% methanol
flow rate 0.2 ml/min
injection 5 µl
detector ABI 3200 QT; ESI(+), MRM (423.3/321.3, 423.3/101.0, 557.3/397.2 and 557.3/453.4)

Description

Categories Analytical Chromatography, Clinical, SPE Applications, Anticholesterolemics, Pharmaceutical & Clinical applications, anticholesterolemics
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for SPE

Materials

     
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