HPLC Analysis of Steroidal Glycosides on Ascentis® Phenyl

HPLC Analysis of Steroidal Glycosides on Ascentis® Phenyl

Conditions

column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium formate (pH 3 with formic acid); [B] methanol
flow rate 1.25 ml/min
sample as indicated in mobile phase A
injection 20 µl
detector ELSD at 45 C, gain 6, 3.5 bar N2

Description

Categories Analytical Chromatography, Steroids
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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