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HPLC Analysis of Steroids on Ascentis® Phenyl

HPLC Analysis of Steroids on Ascentis® Phenyl


column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 3 µm particles (581610-U)
column temp. 35 C
mobile phase [A] water: [B] acetonitrile; (75:25, A:B)
flow rate 1.0 ml/min
sample 50 µg/mL in 85:15, water:methanol
injection 10 µl
detector UV, 240 nm


Categories Analytical Chromatography, Clinical, steroids, Steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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