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HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.)

HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.)

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (60:40, A:B)
flow rate 0.2 ml/min
pressure 2944 psi (203 bar)
sample 50 mg/L in 75:25, water:methanol
injection 2 µl
detector UV, 254 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.
Categories Analytical Chromatography, Clinical, Steroids, steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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