HPLC Analysis of Steroids on Ascentis® Express F5

HPLC Analysis of Steroids on Ascentis® Express F5

Conditions

column Ascentis Express F5, 15 cm x 4.6 mm I.D., 2.7 µm particles (53591-U)
column temp. 35 °C
mobile phase [A] water; [B] methanol; (50:50, A:B)
flow rate 0.8 mL/min
pressure 2393 psi (165 bar)
sample 10 µg/mL of each in 90: 10, water: methanol
injection 5 µL
detector UV, 240 nm

Description

Analysis Note Sample concentrations at 10 ug/mL in 90:10 water:methanol. Acetonitrile does not provide adequate selectivity on this phase. Mass spec was used for peak identification.
Categories Analytical Chromatography, Clinical, steroids, Steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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