HPLC Analysis of Steroids on Ascentis® Express C18

HPLC Analysis of Steroids on Ascentis® Express C18

Conditions

column Ascentis Express C18 10cm x 2.1mm, 2.7 µm particles (53823-U)
column temp. 40 °C
mobile phase [A] water [B] acetonitrile (70:30, A:B)
flow rate 0.7 mL/min
pressure 5438 psi (375 bar)
sample 0.125 mg/mL each in 37.5/62.5 water/organic solvent
injection 2 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Steroids
Featured Industry Clinical
Pharmaceutical (small molecule)
General description This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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