HPLC Analysis of Substituted Poly Aromatic Hydrocarbons using Ascentis® Express Biphenyl

HPLC Analysis of Substituted Poly Aromatic Hydrocarbons using Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 10 cm x 2.1 mm, 2.7 µm particles (64065-U)
column temp. 35 °C
mobile phase [A] Water; [B] Methanol; (30:70 A:B)
flow rate 0.5 mL/min
pressure 6200 psi (427 bar)
sample Uracil (10 µg/mL), Acenaphthenol (250 µg/mL), Acenaphthenone (20 µg/mL),   Acenaphthene (500 µg/mL), Prepared in 70:30 A:B
injection 1 µL
detector UV, 254 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express Biphenyl for the separation of substituted PAHs. It shows the suitability of this phase for separations of extended aromatics and their oxygenated counter parts. These compounds are potentional metabolites, used as synthetic intermediates, and in the study of dihydrodiol dehydrogenase activity in celluar systems.
Categories Analytical Chromatography, PAHs
Featured Industry Chemicals and Industrial Polymers
Environmental
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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