HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series

HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series

Conditions

column Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 µm particles (53970-U)
column temp. 35 C
mobile phase [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate 0.6 ml/min
pressure 812 psi (56 bar)
sample dilute1:9 in acetonitrile
injection 2 µl
detector ELSD, 55 C, 3.5 bar nitrogen

Description

Analysis Note This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.
Categories Analytical Chromatography, Food & Beverage Analysis, Agonist/antagonist, Carbohydrates, HPLC Applications Enzymes, Proteins, Beverage Testing, Organic Acids, Sports and Energy Drinks
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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