HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si


column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (15:85, A:B)
flow rate 1.0 ml/min
injection 10 µl
detector ELSD, 55 C, 3.5 bar nitrogen


Analysis Note This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
Categories Analytical Chromatography, Carbohydrates
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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