HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

Conditions

column Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 µm particles (59602-U)
column temp. 25 °C
mobile phase ([A] 20 mM Tris, pH 8.0 with hydrocloric acid; [B] 20 mM Tris, 0.5 M sodium chloride, pH 8.0 with hydrochloric acid)
gradient 0 to 10% B in 30 min. (linear)
flow rate 0.5 mL/min
injection 10 µL
detector UV, 280 nm

Description

Analysis Note Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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