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HPLC Analysis of Tamoxifen E/Z isomers and Related Compounds on Ascentis® Express RP-Amide

HPLC Analysis of Tamoxifen E/Z isomers and Related Compounds on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 µm particles (53918-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient held at 25% B for 2 min; 25 to 40% B in 15 min; 40 to 25% B in 0.1 min; held at 25% B for 2.9 min
flow rate 0.6 ml/min
pressure 2292 psi (158 bar)
sample 10 mg/L in 10:90, water: methanol
injection 2 µl
detector UV, 260 nm

Description

Categories Analytical Chromatography, Agonist/antagonist
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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